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Active Ras Pull-Down and Detection Kit
Brand: Thermo Fisher ScientificThe Thermo Scientific Active Ras Pull-Down and Detection Kit enables selective enrichment and detection of GTP-bound Ras GTPase through a specific protein interaction with the Raf1 protein-binding domain.
The Active Ras Pull-Down and Detection Kit includes purified GST-Raf1 Ras-binding domain (RBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/washing buffer, anti-Ras antibody, SDS sample buffer, spin columns and collection tubes.
The kit was validated using lysates from NIH 3T3 cells, a cell line known to have robust Ras activity.
Features- Highly sensitive and accurate - optimized reagents, specific anti-Ras antibody and Western blot procedure ensure accurate controls and semi-quantitative results
- Validated - functionally tested for Ras detection to ensure quality and performance
- Compatible - effective for a variety of cell types from mouse, rat and human sources
1 - 1 of 1 Part Numbers
16117
Kit Contents:
Box 89855X (these items ship together on dry ice; upon receipt store at -70°C):
GST-Raf1-RBD, 2.4mg, contains 2-3mg/mL in 50mM Tris•HCl, pH 7.2, 150mM NaCl, 0.5% Triton® X-100, 5mM MgCl2, 1mM DTT and 10% glycerol; GST-Raf1-RBD interacts with Ras from human and mouse, and possibly from all mammalian species, store at -70°C
100X GTPyS, 50µL, 10mM in sterile water, store at -70°C (or -20°C)
100X GDP, 50µL, 100mM in sterile water, store at -70°C (or -20°C)
Box 89855Y (these items ship together with an ice pack; upon receipt store at 4°C):
Anti-Ras Antibody, 250µL (5 units) mouse monoclonal Ig2ak; Anti-Ras antibody reacts with Ras of human, rat, and mouse; store at 4°C. Note: One unit of Anti-Ras antibody is defined as the amount of antibody required to efficiently detect Ras in 20µg NIH3T3 whole cell lysate by Western blotting (8.5 x 7.5cm membrane).
Glutathione Resin, 3.0mL, supplied as 50% slurry containing 0.05% sodium azide, store at 4°C
1X Lysis/Binding/Wash Buffer, 100mL, contains 25mM Tris•HCl, pH 7.2, 150mM NaCl, 5mM MgCl2, 1% NP-40 and 5% glycerol, store at 4°C
2X SDS Sample Buffer, 1.5mL, contains 125mM Tris•HCl, pH 6.8, 2% glycerol, 4% SDS (w/v) and 0.05% bromophenol blue, store at 4°C
Spin Cups, 30 each, maximum volume 850µL, store at room temperature or 4°C
Collection Tubes, 90 each, store at room temperature or 4°C
1 - 1 of 1 Part Numbers
Specifications
Brand
Assay
- Protein Quantitation Assay
Substrate Properties
- Protein-based Substrate
Substrate Type
- GTPase Substrate
Target
- GTPases
- ATPases
- Phosphodiesterases
- Kinases (Multiple)
- Phosphatases
Contents & Storage
- Lysis/Binding/Wash Buffer (1X), 100 mL
- SDS Sample Buffer (2X), 1.5 mL
- GTPyS (100X), 50 µL
- GDP (100X), 50 µL
- Glutathione Agarose Resin, 3 mL
- Anti-Ras Antibody, 5 units, 250 µL
- Spin Cups, 30 cups
- GST-Raf1-RBD Fusion Protein, 1 vial
- Collection Tubes, 90 Tubes