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Active Ras Pull-Down and Detection Kit

Brand: Thermo Fisher Scientific

The Thermo Scientific Active Ras Pull-Down and Detection Kit enables selective enrichment and detection of GTP-bound Ras GTPase through a specific protein interaction with the Raf1 protein-binding domain.

The Active Ras Pull-Down and Detection Kit includes purified GST-Raf1 Ras-binding domain (RBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/washing buffer, anti-Ras antibody, SDS sample buffer, spin columns and collection tubes.

The kit was validated using lysates from NIH 3T3 cells, a cell line known to have robust Ras activity.
Features
  • Highly sensitive and accurate - optimized reagents, specific anti-Ras antibody and Western blot procedure ensure accurate controls and semi-quantitative results
  • Validated - functionally tested for Ras detection to ensure quality and performance
  • Compatible - effective for a variety of cell types from mouse, rat and human sources

1 - 1 of 1 Part Numbers
16117
16117
THERMO FISHER Active Ras Pull-Down and Detection Kit, Contains Sufficient Reagents for 30 Pull-down Reactions
Kit Contents: 

Box 89855X (these items ship together on dry ice; upon receipt store at -70°C): 

GST-Raf1-RBD, 2.4mg, contains 2-3mg/mL in 50mM Tris•HCl, pH 7.2, 150mM NaCl, 0.5% Triton® X-100, 5mM MgCl2, 1mM DTT and 10% glycerol; GST-Raf1-RBD interacts with Ras from human and mouse, and possibly from all mammalian species, store at -70°C 

100X GTPyS, 50µL, 10mM in sterile water, store at -70°C (or -20°C) 

100X GDP, 50µL, 100mM in sterile water, store at -70°C (or -20°C) 

Box 89855Y (these items ship together with an ice pack; upon receipt store at 4°C): 

Anti-Ras Antibody, 250µL (5 units) mouse monoclonal Ig2ak; Anti-Ras antibody reacts with Ras of human, rat, and mouse; store at 4°C. Note: One unit of Anti-Ras antibody is defined as the amount of antibody required to efficiently detect Ras in 20µg NIH3T3 whole cell lysate by Western blotting (8.5 x 7.5cm membrane). 

Glutathione Resin, 3.0mL, supplied as 50% slurry containing 0.05% sodium azide, store at 4°C 

1X Lysis/Binding/Wash Buffer, 100mL, contains 25mM Tris•HCl, pH 7.2, 150mM NaCl, 5mM MgCl2, 1% NP-40 and 5% glycerol, store at 4°C 

2X SDS Sample Buffer, 1.5mL, contains 125mM Tris•HCl, pH 6.8, 2% glycerol, 4% SDS (w/v) and 0.05% bromophenol blue, store at 4°C 

Spin Cups, 30 each, maximum volume 850µL, store at room temperature or 4°C 

Collection Tubes, 90 each, store at room temperature or 4°C
1 - 1 of 1 Part Numbers

Specifications

Assay

  • Protein Quantitation Assay

Substrate Properties

  • Protein-based Substrate

Substrate Type

  • GTPase Substrate

Target

  • GTPases
  • ATPases
  • Phosphodiesterases
  • Kinases (Multiple)
  • Phosphatases

Contents & Storage

  • Lysis/Binding/Wash Buffer (1X), 100 mL
  • SDS Sample Buffer (2X), 1.5 mL
  • GTPyS (100X), 50 µL
  • GDP (100X), 50 µL
  • Glutathione Agarose Resin, 3 mL
  • Anti-Ras Antibody, 5 units, 250 µL
  • Spin Cups, 30 cups
  • GST-Raf1-RBD Fusion Protein, 1 vial
  • Collection Tubes, 90 Tubes

Documentation

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